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Monosaccharide Mass and Low Mass MS/MS (CID) Carbohydrate Marker Ions

 

Monosaccharide /
Disaccharide

Composition

Monoisotopic mass

Low Mass CID Marker Ions
Intact Residue
Hexose, Hex
(Galactose, Mannose)
C6O6H12 180.0634 162.0528 163
Deoxy-Hexose, dHex
(Fucose)
C6O5H12 164.0685 146.0579 147
N-acetylhexoseamine, HexNAc
(N-acetylglucoseamine,
N-acetylgalactoseamine)
 
C8O6NH15 221.0899 203.0794 204
N-acetylneuraminic
acid
, Neu5Ac, NANA
(sialic acid)
C11O9NH19 309.1060 291.0954 292/274/256
Hex-HexNAc C14O11NH25 383.1428 365.1322 366
Hex3HexNac2
 (Common N-linked Core)
C46O35N2H76 910.3278 892.3172 -
Reference: (1) Conboy, J. J. and Henion, J. D., The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation, JASMS, 3, 804,1992. (2) Huddleston, M. J., Bean, M. F., Carr, S. A., Collisional fragmentation of
glycopeptides by electrospray ionization LC/MS and LC/MS/MS. Methods for selective detection of glycopeptides in protein digests, Anal. Chem., 65, 877, 1993.

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Last updated:  Tuesday, January 19, 2016 02:49:08 PM