Mass Spectrometry Standards at IonSource

return to standards index | return to IonSource

Horse Myoglobin

 

 

Expasy Horse Myoglobin Page.

Accession # P68082

Fasta Format

>P68082|MYG_HORSE Myoglobin - Equus caballus (Horse).
MGLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE
DLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKH
PGDFGADAQGAMTKALELFRNDIAAKYKELGFQG
Download Horse Myoglobin PIR file for use with IsoPro 3.0
(right click and save to disk, make sure extension is .pir)
Elemental Composition:	C769 H1212 N210 O218 S2 
Monoistopic Mass		16940.96 (desMet)
Average Mass		16951.48  (desMet)
pI			7.36
 
 
Trypsin Digest











	

		

			

				

					
						
						
					
					

						Horse Myoglobin
						
						
ESI Charge States

					

					

						
						
						
						
Monoisotopic Mass 

					

					

						
						
						 
						 
						 
						 
					

					

						peptide sequence
						1Z
						2Z
						3Z
						4Z
					

					

						K
						147.1128
						 
						 
						 
					

					

						HK
						284.1717
						 
						 
						 
					

					

						FK
						294.1812
						 
						 
						 
					

					

						YK
						310.1761
						 
						 
						 
					

					

						HLK
						397.2558
						 
						 
						 
					

					

						FDK
						409.2081
						205.1041
						 
						 
					

					

						IPIK
						470.3337
						235.6669
						 
						 
					

					

						NDIAAK
						631.3409
						316.1705
						 
						 
					

					

						ELGFQG
						650.3144
						325.6572
						 
						 
					

					

						ASEDLK
						662.3355
						331.6678
						 
						 
					

					

						TEAEMK
						708.3232
						354.6616
						 
						 
					

					

						ALELFR
						748.4352
						374.7176
						 
						 
					

					

						LFTGHPETLEK
						1271.6630
						636.3315
						424.5543
						318.6658
					

					

						HGTVVLTALGGILK
						1378.8416
						689.9208
						460.2805
						345.4604
					

					

						HPGDFGADAQGAMTK
						1502.6692
						751.8346
						501.5564
						376.4173
					

					

						VEADIAGHGQEVLIR
						1606.8547
						803.9274
						536.2849
						402.4637
					

					

						GLSDGEWQQVLNVWGK
						1815.9024
						908.4512
						605.9675
						454.7256
					

					

						GHHEAELKPLAQSHATK
						1853.9616
						927.4808
						618.6539
						464.2404
					

					

						YLEFISDAIIHVLHSK
						1885.0218
						943.0109
						629.0073
						472.0055
					

					

						
						
						 
						 
						 
						 
					

					

						
						oxized variants and conflicts
						 
						 
						 
						 
					

					

						TEAEMK (ox)
						724.3182
						362.6591
						242.1061
						181.8296
					

					

						HPGNFGADAQGAMTK (con, 
			D/N)
						1501.6842
						751.3421
						501.2281
						376.1711
					

					

						HPGDFGADAQGAMTK (ox)
						1518.6641
						759.8321
						506.8880
						380.4160
					

					

						
						
						 
						 
						 
						 
					

					

						
						
						 
						 
						 
						 
					

					

						Note: m/z values in blue are commonly observed 
			in nano ESI.
					

				

			

		

	







	

		

			
 

			
Typical Myoglobin Peptide Map 
			(follow link)

			
Myoglobin Digest Procedure
			(follow link)

		

		
 

		

			
References: 

		

		
    
			
  1. 
			Zaia J, Annan RS, Biemann K.
    The correct molecular weight of myoglobin, a common calibrant for 
			mass spectrometry.
    
			Rapid 
			Commun Mass Spectrom. 1992 Jan;6(1):32-6. 
     
    2. 
			
  2. Our vendor of choice for this standard is 
			
			
			Sigma-Aldrich  
			60 nmol vials of horse myoglobin 
			Sigma-Aldrich product number A8673-1VL and  A8673-5X1VL 
			. Sigma-Aldrich web
			reference
    3. 
		

	

	
 




 


 



	

	

		

			
			
home 
			| disclaimer

			Copyright © 2000-2016  IonSource.Com  All rights reserved. 

			Last updated: 
			Tuesday, January 19, 2016 02:49:53 PM