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Principles of MS Quantitation
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Table of Contents:
Introduction to MS Quantitation
Total Ion Current (TIC)
Selected Ion Monitoring (SIM)
Selected Reaction Monitoring (SRM / MRM)
Development of an SRM Transition
The Internal Standard
PK/LC/MS/MS Reverse Phase Chromatography Method Development
Sample Preparation
Standards and Standard Curve Preparation
Standard Curve Creation Procedure
Example Standard Curve Dilution Scheme
Running the Standard Curve
Randomizing Standards and Samples
Running System Suitability
The "QCs"
Links to PK Resources
Quantitation Tips
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