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Glycopeptide Heterogeneity and Mass Pattern Recognition

 

 

 

Sugar Composition +1 +2 +3 +4 +5
dHexose C6O4H10 146.1 73 48.7 36.5 29.2
Hexose C6O5H10 162.1 81.0 54.0 40.4 32.4
HexNAc C8O5NH13 203.2 101.6 67.7 50.8 40.6
NANA C11O8NH17 291.3 145.6 97.1 72.8 58.2
HexNac+Hex C14O10NH23 365.3 182.6 121.8 91.3 73.1

 

 

Example ESI Mass Spectrum

Above is a sample electrospray mass spectrum showing peaks that differ by 54 amu this is typical of a glycopeptide displaying mannose type glycosylation heterogeneity at the [M+3H]3+ charge state (see the chart above).  To confirm the observation look for peaks that differ by 40.4 amu in the lower end of the spectrum indicating a quadruply charged series.

 

Monosaccharide table with structures

Simple monosaccharide mass table Low mass CID marker ions

 

References

IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN), Nomenclature of glycoproteins, glycopeptides and peptidoglycans glycopeptides and peptidoglycans glycopeptides and peptidoglycans, Recommendations 1985.

 

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Last updated:  Tuesday, January 19, 2016 02:48:56 PM