An IonSource Tutorial Monograph 0004 

S-Carboxymethylation of Cysteine ..Method References and Cool Papers: 
(***abstract available)
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S-Carboxymethylation Papers

  1. Crestfield A M; Moore S; Stein W H. J. Biol. Chem. 1963; 238, 622
    (the seminal iodoacetic acid alkylation paper)

  2. Raftery M A; Cole R D.  On the aminoethylation of proteins. J. Biol. Chem. 1966; 241, 3457-3461.

  3. Brune D C Alkylation of cysteine with acrylamide for protein sequence analysis.
    Anal Biochem. 1992  Dec;207(2):285-90.  PMID: 1481983; UI: 93128583

  4. Ruegg U T; Rudinger J.   Reductive cleavage of cysteine disulfides with tributylphosphine.  Meth. Enzymol. 1977  47, 111-116.

  5. Amons R. Vapor-phase modification of sulfhydryl groups in proteins. FEBS Letters. 1987   212, 68-72


    Cool Papers and Books

  6. Adamczyk M, Gebler JC, Wu J.  A simple method to identify cysteine residues by isotopic labeling and ion trap mass spectrometry. Rapid Commun Mass Spectrom. 1999;13(18):1813-7. PMID: 10482894; UI: 99414092. ***

  7. Meredith MJ. Analysis of protein-glutathione mixed disulfides by high performance liquid chromatography.  Anal Biochem. 1983 Jun;131(2):504-9.  PMID: 6614484; UI: 83306571***

  8. Kellner R; Lottspeich F; Meyer H E.  Microcharacterization of Proteins. 1994. publisher VCH.  ISBN 3-527-30048-1


    ABRF Links

  9. Alkylation of Cysteine, ABRF forum archive

  10. Storage of iodoacetic acid, ABRF forum archive

 



 

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