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- De Novo Peptide Sequencing Tutorial

IonSource.Com

pI plotting

This table was generated using the ExPASy "Compute pI/Mw tool"

http://www.expasy.org/tools/pi_tool.html

Here is the pI graph generated from the table above.

Notice the y ion intensity takes a hit when we encounter glutamic acid, going from y₁₀ to y₁₁ and then again when we cross aspartic acid going from y₁₃. 

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Reference Figures

b and y Ion Table

This table was generated using the ISB tool,
found here.
http://db.systemsbiology.net:8080/proteomicsToolkit/FragIonServlet.html

Full Fragment Spectrum